Technical Knowledge Base
Application Notes
- ★ Bz-Thiol-dT Linker: History and Applications
- ★ Colourimetric Detection & Capture using Biotin Products
- ★ Oligo Spacers: Your Flexible Friends
- ★ Why are there so many amino and thiol linkers?
- Use of dPEG® Spacers in PNA Synthesis
- Application of Reverse (5’ to 3’) Oligonucleotides
Protocols
- ★ DNP Labelling
- ★ DNA Damage/Repair - Epigenetics Products
- ★ Glyceryl Modification
- ★ Use of CAL Fluor® Dyes
- Using Non-Fluorescent (Dark) Quenchers - BlackBerry® Quenchers
- Aldehyde Modification
FAQs
- ★ Do you have any suggestions for DNA modifications that increase the (long-term) stability in target cells?
- What is AMA and what is its advantage over ammonium hydroxide as a deprotection solution?
- When I make amino-modified oligos for post-labelling I get very little yield and see a oligo mass + 53 in the mass spec. What is this and how can I stop this from happening?
- Why must I use DCM to dissolve 5'-cholesterol or 5'-tocopherol when acetonitrile works for 5'-cholesterol-TEG?
- What is the difference between the types of purification cartridge?
- Will t-butylamine/MeOH/water 1:1:2, 2h, 70oC work for the deprotection of a HEX/TAMRA probe?
Posters and Presentations
- ★ Vitamin Based Phosphoramidites for use as Potential Oligonucleotide Delivery Reagents
- ★ New Potential Oligonucleotide Delivery Reagents
- Plant Derived Cholesterol Modifications - A Comparison of Commercially Available Cholesterol Phosphoramidites and Solid Supports for use in Oligonucleotide Synthesis of DNA and RNA (TC and TBDMS chemistries)
- Compatibility of TC-RNA Chemistry with Common Modifications
- A Comparison of Hydrophilic and Hydrophobic Amino and Thiol Linkers for use in Post-Synthetic Labelling of Oligonucleotides
- The Synthesis of Ribozymes using TC-RNA Chemistry
Announcements
Product News
- ★ CAL Fluor® Dyes
- ★ bisDMT-Pyridoxine-C6-CE Phosphoramidite
- 5'-TFA-Amino-Modifier-C5-CE Phosphoramidite
- 5'-TFA-Amino-Modifier-C12-CE Phosphoramidite
- 5'-Niacin-CE Phosphoramidite
- 5-Me-dC(Ac)-CE Phosphoramidite