Applicable Products
2098 | 2'-OMe-I-CE Phosphoramidite |
2099 | 2'-OMe-5-Me-U-CE Phosphoramidite (2'-OMe-T) |
2192 | 2'-OMe-N-Ac-5-Me-C-CE Phosphoramidite |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
2098 | C41H49N6O8P | 784.85 | 344.22 | 3.19 | 6.37 | 12.74 |
2099 | C41H51N4O9P | 774.85 | 334.23 | 3.23 | 6.45 | 12.91 |
2192 | C43H54N5O9P | 815.90 | 333.24 | 3.06 | 6.13 | 12.26 |
Coupling
ETT, BTT or DCI can be used as activators. A coupling time of 6min (360s) is recommended.
Oxidation
No changes are required from standard methods.
Cleavage & Deprotection
Standard oligonucleotide deprotection conditions can be applied when deprotecting an oligo containing these modifications. In general, AMA at 65ºC for 10min is used, employing dmf-G and Ac-C in the sequence.
Storage & Stability
Store in a freezer below -10°C. Diluted samples are freshly prepared and used within 24h.
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