Applicable Product
| 2167 | Ferrocene-dT-CE Phosphoramidite |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
| 2167 | C60H73N6O10PFe | 1125.09 | 684.46 | 2.22 | 4.44 | 8.89 |
Coupling
A 5min coupling time is recommended. Ac-dC and dmf-dG must be used in the oligo synthesis to allow AMA deprotection.
Cleavage & Deprotection
Cleave and deprotect using AMA for 10min at 65˚C, followed by immediate desalting with G25 to remove the deprotection solution.
Purification
Best results are obtained using ion-exchange HPLC. Note to avoid oxidation of the ferrocene ring system it is recommended all solvents and buffers are deoxygenated with argon.
Storage & Stability
Store in a freezer at -10 to -30˚C. Acetonitrile solutions must be used within 24h.
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