Applicable Products
| 2009 | 5-I-dC-CE Phosphoramidite |
| 2010 | 5-F-dU-CE Phosphoramidite |
| 2011 | 5-Br-dC-CE Phosphoramidite |
| 2012 | 5-Br-dU-CE Phosphoramidite |
| 2014 | 5-I-dU-CE Phosphoramidite |
| 2054 | 8-Br-dA-CE Phosphoramidite |
| 2055 | 8-Br-dG-CE Phosphoramidite |
| 2325 | 5-Br-dU SynBase™ CPG 1000/110 |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
| 2009 | C46H51N5O8PI | 959.83 | 415.08 | 2.60 | 5.21 | 10.42 |
| 2010 | C39H46N4O8PF | 748.79 | 308.16 | 3.34 | 6.68 | 13.35 |
| 2011 | C46H51N5O8PBr | 912.82 | 368.08 | 2.74 | 5.48 | 10.96 |
| 2012 | C39H46N4O8PBr | 809.69 | 369.07 | 3.09 | 6.18 | 12.35 |
| 2014 | C39H46N4O8PI | 856.69 | 416.07 | 2.92 | 5.84 | 11.67 |
| 2054 | C43H52N8O6PBr | 887.81 | 392.11 | 2.82 | 5.63 | 11.26 |
| 2055 | C43H52N8O7PBr | 903.90 | 408.10 | 2.77 | 5.53 | 11.06 |
| 2325 | - | - | 369.07 | - | - | - |
Coupling
No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites and supports.
Deprotection
To avoid ammonolysis at the halogenated site, a mild deprotection step is recommended using ammonium hydroxide for 24h at room temperature, or AMA for 2h at room temperature provided dmf-dG and Ac-dC are used. Bz-protected C amidites are not compatible with AMA deprotection.
Storage & Stability
Store the solid phosphoramidites refrigerated, dry, at a maximum of 2-8°C. Store the monomers in solution for no longer than 24h, although 8-Br-dG (2055) and 5-Br-dU (2012) are stable for 2-3 days. Halogenated nucleosides tend to be photosensitive therefore retain the amidites in amber vials.
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