Applicable Products
| 2072 | 8-oxo-dG-CE Phosphoramidite |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
| 2072 | C44H54N7O9P | 855.93 | 345.21 | 2.92 | 5.84 | 11.68 |
Coupling
No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.
Deprotection
Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol for 17h at 55°C to avoid oxidative degradation of the 8-oxo-dG site.
Storage & Stability
Store in the fridge, dry. Stability in solution is 24h.
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