Applicable Products
| 2350 | 3'-Amino-Modifier-C7 CPG 1000 |
| 2365 | 3'-PT-Amino-Modifier-C6 CPG |
| 2367 | 3'-Amino-Modifier-C6-dT CPG |
| 2369 | 3'-Amino-Modifier-C6-dC CPG |
| 2371 | 3'-PT-Amino-Modifier-C3 CPG |
Physical Data
Item |
Unit Wt. |
| 2350 | 209.18 |
| 2365 | 179.15 |
| 2367 | 359.24 |
| 2369 | 457.42 |
| 2371 | 137.07 |
Coupling
All supports are used as per unmodified nucleoside supports. However, non-nucleosidic modifications are slow to detritylate and require an initial detritylation prior to use in synthesis. In this case it is important not to use a cycle with an initial capping step. AMA deprotection will require dmf-G and Ac-C to be used.
Cleavage & Deprotection
2350—Cleavage of the oligonucleotide requires 2h at room temperature with ammonium hydroxide or AMA. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection. Alternatively, 20% piperidine in DMF can be used, rather than DEA. In fact this will simultaneously remove Fmoc and cyanoethyl protection, plus remove the vinylfluorene by-product from the Fmoc deprotection reaction.
For the PT-Amino CPGs (2371 & 2365)—Cleavage of the oligonucleotide requires overnight at 55°C with ammonium hydroxide. This treatment will complete the deprotection of the nucleobases for all protection types. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection.
2369 & 2367—Use deprotection conditions as required by the nucleobases. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection.
Storage & Stability
The supports are stored in a freezer at –10 to –30°C.
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