Will t-butylamine/MeOH/water 1:1:2, 2h, 70oC work for the deprotection of a HEX/TAMRA probe?


Unfortunately, the deprotection of HEX and TAMRA are not compatible therefore one of these modifications has to be added post synthetically. For instance using 3'-Fmoc protected amino C7 CPG (2350) along with HEX amidite (2136) will allow deprotection in ammonium hydroxide and the TAMRA is added to the aminolinker using TAMRA NHS ester (0251).


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