What is AMA and what is its advantage over ammonium hydroxide as a deprotection solution?


AMA is a 1:1 mixture of 28% (or higher) ammonium hydroxide and 40% aqueous methylamine used to deprotect oligos synthesised with fast deprotection amidites (d/rABz, d/rGdmf, d/rCAc). This shortens the deprotection time to 10min at 65oC compared with 2-3h in ammonium hydroxide at the same temperature.


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