5'-Palmitate-C6-CE Phosphoramidite (2199) is now available.
Labelling oligonucleotides with lipophilic compounds such as cholesterol as a means of improving cell penetration is well known. Link offers a range of oligonucleotide synthesis reagents for use in this field and the cholesterol and tocopherol modifiers have proved particularly successful.
Subsequently we are developing new reagents we believe have the potential to aid the delivery of oligonucleotides into a cell. One of the new reagents is a fatty acid derivative of palmitic acid; 5’-Palmitate-C6-CE Phosphoramidite. This is incorporated at the 5’-end of the oligonucleotide using standard methods.
Palmitic acid is the most commonly utilised fatty acid found in the body. It is the first such acid produced during fatty acid synthesis and is the precursor to all others. This is known to conjugate to cysteine residues in proteins, in particular membrane proteins, and is thought to aid the movement of proteins between compartments in the cell. Similarly, palmitic acid has been conjugated to BSA which can then be absorbed and utilised by the cell. Oligo-3’-palmitate conjugates have been shown to be potential telomerase inhibitors.
A 5’-palmitate modifier is a logical addition to our range of products and complements our existing 3’-Palmitate CPG product.