|2326||3'-Glyceryl SynBase CPG 1000|
As with all non-nucleosidic supports, an additional deblock step is required.
Cleavage & Deprotection
This is dependent on the nucleobase protection and other modifications but is typically ammonium hydroxide at 55ºC for 4h, or AMA at 65ºC for 10min.
Storage & Stability
Store dry in a freezer at –10 to –30°C.
- For oxidation and reduction methods for converting the glycerol group, see: Convenient synthesis of oligonucleotides with a 3’-phosphoglycolate and 3’-phosphoglycaldehyde terminus, H. Urata and M. Akagi, Tetrahedron Lett., 34, 4015-4018 (1993). [Abtract]